Making Stickleback Crosses

Multiple researchers have Protocols.io DOIs with Stickleback Cross protocols.

Stickleback IVF includes pictures of spoiled, unfertilized, and poor-quality eggs for reference.

For this protocol, females can be anesthetized for egg removal, but the males will be sacrificed for access to their gonads and sperm.

Included here are methods of doing so, materials needed, and a basic timeline of what to expect from the zygotes post fertilization until hatch.

Seen: Hatchling stickleback minutes past hatching, some in the midst of hatching, and one still in the chorion